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81.
Antonio Marinho da Silva Neto Samuel Reghim Silva Michele Vendruscolo Carlo Camilloni Rinaldo Wander Montalvão 《Proteins》2019,87(4):302-312
Here a differential geometry (DG) representation of protein backbone is explored on the analyses of protein conformational ensembles. The protein backbone is described by curvature, κ, and torsion, τ, values per residue and we propose 1) a new dissimilarity and protein flexibility measurement and 2) a local conformational clustering method. The methods were applied to Ubiquitin and c-Myb-KIX protein conformational ensembles and results show that κ\τ metric space allows to properly judge protein flexibility by avoiding the superposition problem. The dmax measurement presents equally good or superior results when compared to RMSF, especially for the intrinsically unstructured protein. The clustering method is unique as it relates protein global to local dynamics by providing a global clustering solutions per residue. The methods proposed can be especially useful to the analyses of highly flexible proteins. The software written for the analyses presented here is available at https://github.com/AMarinhoSN/FleXgeo for academic usage only. 相似文献
82.
Cecilia Nigro Alessia Leone Michele Longo Immacolata Prevenzano Thomas H. Fleming Antonella Nicolò Luca Parrillo Rosa Spinelli Pietro Formisano Peter P. Nawroth Francesco Beguinot Claudia Miele 《生物化学与生物物理学报:疾病的分子基础》2019,1865(1):73-85
Impaired angiogenesis leads to long-term complications and is a major contributor of the high morbidity in patients with Diabetes Mellitus (DM). Methylglyoxal (MGO) is a glycolysis byproduct that accumulates in DM and is detoxified by the Glyoxalase 1 (Glo1). Several studies suggest that MGO contributes to vascular complications through mechanisms that remain to be elucidated. In this study we have clarified for the first time the molecular mechanism involved in the impairment of angiogenesis induced by MGO accumulation.Angiogenesis was evaluated in mouse aortic endothelial cells isolated from Glo1-knockdown mice (Glo1KD MAECs) and their wild-type littermates (WT MAECs). Reduction in Glo1 expression led to an accumulation of MGO and MGO-modified proteins and impaired angiogenesis of Glo1KD MAECs. Both mRNA and protein levels of the anti-angiogenic HoxA5 gene were increased in Glo1KD MAECs and its silencing improved both their migration and invasion. Nuclear NF-?B-p65 was increased 2.5-fold in the Glo1KD as compared to WT MAECs. Interestingly, NF-?B-p65 binding to HoxA5 promoter was also 2-fold higher in Glo1KD MAECs and positively regulated HoxA5 expression in MAECs. Consistent with these data, both the exposure to a chemical inhibitor of Glo1 “SpBrBzGSHCp2” (GI) and to exogenous MGO led to the impairment of migration and the increase of HoxA5 mRNA and NF-?B-p65 protein levels in microvascular mouse coronary endothelial cells (MCECs).This study demonstrates, for the first time, that MGO accumulation increases the antiangiogenic factor HoxA5 via NF-?B-p65, thereby impairing the angiogenic ability of endothelial cells. 相似文献
83.
Canovetti Annalisa Rossi Francesca Rossi Michele Menabuoni Luca Malandrini Alex Pini Roberto Ferrara Paolo 《Biomechanics and modeling in mechanobiology》2019,18(2):319-325
Biomechanics and Modeling in Mechanobiology - The aim of this study was to qualitatively evaluate the biomechanical load resistance of different surgical wound configurations (mushroom, zig-zag,... 相似文献
84.
Valentinuzzi Fabio Venuti Silvia Pii Youry Marroni Fabio Cesco Stefano Hartmann Felix Mimmo Tanja Morgante Michele Pinton Roberto Tomasi Nicola Zanin Laura 《Plant molecular biology》2019,101(1-2):129-148
Plant Molecular Biology - Iron and phosphorus are abundant elements in soils but poorly available for plant nutrition. The availability of these two nutrients represents a major constraint for... 相似文献
85.
Christopher W. Schmidt Ashley Remy Rebecca Van Sessen John Willman Kristin Krueger Rachel Scott Patrick Mahoney Jeremy Beach Jaqueline McKinley Ruggero D'Anastasio Laura Chiu Michele Buzon J. Rocco De Gregory Susan Sheridan Jacqueline Eng James Watson Haagen Klaus Pedro Da-Gloria Jeremy Wilson Abigail Stone Paul Sereno Jessica Droke Rose Perash Christopher Stojanowski Nicholas Herrmann 《American journal of physical anthropology》2019,169(2):207-226
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87.
Colin Umeda Michele Eatough Jones Timothy Paine 《Entomologia Experimentalis et Applicata》2019,167(9):820-825
Polyphagous shot hole borer (PSHB), Euwallacea whitfordiodendrus (Schedl) (Coleoptera: Curculionidae, Scolytinae), is an ambrosia beetle that has recently invaded southern California, USA. This beetle successfully attacks and reproduces in a multitude of tree species. As direct control methods are limited, we investigated cultural management options, and sought to determine whether irrigation affects the number of attacks host trees experienced. If irrigation plays a role, cultural control methods could be recommended to managers and growers. Three separate experiments were conducted that monitored the number of attacks on trees with different levels of irrigation. Two experiments examined PSHB attacks in established landscape trees where irrigation was either present or absent. A third experiment used young potted box elder with irrigation controlled with timed emitters. In all three experiments, the level of irrigation received by the trees did not affect the number of attacks. The results suggest that changes in irrigation practices do not affect risk from PSHB attack. 相似文献
88.
Claudia Abbruzzese Silvia Matteoni Michele Persico Barbara Ascione Silvia Schenone Francesca Musumeci Rosario Amato Nicola Perrotti Paola Matarrese Marco G. Paggi 《Journal of cellular physiology》2019,234(12):22529-22542
The small molecule SI113 is an inhibitor of the kinase activity of SGK1, a key biological regulator acting on the PI3K/mTOR signal transduction pathway. Several studies demonstrate that this compound is able to strongly restrain cancer growth in vitro and in vivo, alone or in associative antineoplastic treatments, being able to elicit an autophagic response, either cytotoxic or cytoprotective. To elucidate more exhaustively the molecular mechanisms targeted by SI113, we performed activity-based protein profiling (ABPP) proteomic analysis using a kinase enrichment procedure. This technique allowed the identification via mass spectrometry of novel targets of this compound, most of them involved in functions concerning cell motility and cytoskeletal architecture. Using a glioblastoma multiforme, hepatocarcinoma and colorectal carcinoma cell line, we recognized an inhibitory effect of SI113 on cell migration, invading, and epithelial-to-mesenchymal transition. In addition, these cancer cells, when exposed to this compound, showed a remarkable subversion of the cytoskeletal architecture characterized by F-actin destabilization, phospho-FAK delocalization, and tubulin depolimerization. These results were definitely concordant in attributing to SI113 a key role in hindering cancer cell malignancy and, due to its negligible in vivo toxicity, can sustain performing a Phase I clinical trial to employ this drug in associative cancer therapy. 相似文献
89.